Effect of glycerolipid preparations from fern and horsetail on human peripheral blood mononuclear cells under ex vivo conditions

Introduction. Human peripheral blood mononuclear cells (PBMCs) are a pool of immune and they also convenient model system for studying pathologies. Aim. Testing bioactivity glycerolipid prepa­rations from fern horsetail species containing long chain polyunsaturated fatty acids (LCPUFAs) towards PBMCs without exogenous stimulation after phorbol-12-myristate-13-acetate (PMA) plus ionomycin stimulation. Materials methods. Glycerolipid preparations were produced by fractionation total lipids, isolated young fronds the Matteuccia struthiopteris shoots Equisetum arvense , on silica. Egg phosphatidylcholine was used comparison. Fatty analyzed gas chromatography. Mononuclear patients with asthma. Parameters cell viability activation estimated flow cytometry. Results. The prep­arations found to have cytotoxic effect while egg not. most active fraction lipids eluted methanol which reduced 64.6 (51.1-79.0)% in concentration 2 pg/ml caused complete death 20 pg/ml. After PMA/ionomycin, cyto­toxic preparation increased although level expressing marker CD69 did not change. other observed higher concentrations (20 and/or 80 pg/ml) it less pronounced: 7.1 (6.7-9.4)% mixture chlo­roform - water (3:5:2), 39.8 (26.4-41.6)% 12.0 (10.0-15.5)% fractions chloroform-methanol-water mixture, respectively, Conclu­sion. Comparison acid composition confirm contribution LCPUFAs effects. Identification an component may allow development drug local application hyperimmune response or experiments.